Journal: Oncotarget

Article Title: Estrogen promotes tumor metastasis via estrogen receptor beta-mediated regulation of matrix-metalloproteinase-2 in non-small cell lung cancer

doi: 10.18632/oncotarget.16992

Figure Lengend Snippet: (A) The migration in wound-healing assay of A549 cells after treatment with DMSO or E2 (10 nM), PPT (10 nM), DPN (10 nM), E2+Ful (1 μM) and Ful (1 μM) for 24 h (magnification 40×); (B) The effect on wound closure (percentage) in A549 cells ( *p < 0.05 vs. control group, #p < 0.05 vs. E2 group ); (C) Representative images of Transwell assays to assess A549 cell invasion and migration; (D) The effect of transwell migration assays in A549 cells after treating with DMSO or E2 (10 nM), PPT (10 nM), DPN (10 nM), E2+Ful (1μM) and Ful(1μM) for 24 h; The effect of transwell migration assays in H1793 cells after treatment with the same drugs for 72 h; (E) Representative images of Transwell assays for assessment of H1793 cell invasion and migration; (F) The effect of transwell migration assays in H1793 cells after treatment with DMSO or E2 (10 nM), PPT (10 nM), DPN (10 nM), E2+Ful (1 μM) and Ful (1 μM) for 24 h. The effect of transwell migration assays in H1793 cells after treatment with the same drugs for 72 h; (G-H) Western blot analysis of ERβ, MMP-2, MMP-9, p-p38MAPK, p38MAPK, pAKT and tAKT protein levels at 48 h in A549 cells, respectively. GAPDH was used as a control. All data are shown as the mean ± SD. Results represent three independent experiments. Student's t-test was carried out ( *p < 0.05 vs. control group, #p < 0.05 vs. E2 group ).

Article Snippet: The antibodies used during Western blots included rabbit anti-human ERα from Proteintech, rabbit anti-human ERβ (1:500) from Proteintech, mouse anti-human MMP-1(1:400) from Santa Cruz, mouse anti-human MMP-2(1:400) from Santa Cruz, rabbit anti-human MMP-9 (1:500) from Proteintech, rabbit anti-human p38MAPK(1:500)/p-p38MAPK(1:500) and rabbit anti-human tAkt (1:500)/pAkt(1:500) from Bioworld Technology.

Techniques: Migration, Wound Healing Assay, Western Blot

Journal: Oncotarget

Article Title: Estrogen promotes tumor metastasis via estrogen receptor beta-mediated regulation of matrix-metalloproteinase-2 in non-small cell lung cancer

doi: 10.18632/oncotarget.16992

Figure Lengend Snippet: (A) Western blot analysis of protein expression of ERβ, MMP-2, p-p38MAPK, p38MAPK, pAKT and tAKT in NSCLC cell line A549 when treating with DMSO or E2 (10 nM), E2+LY294002 (10 nM), LY294002 (10 nM), E2+SB203580 (10 nM) and SB203590 (10 nM) for 48 h. (B) the detection of optical density. GAPDH was used as a control. All data are shown as the mean ± SD. Results represent three independent experiments. Student's t-test was carried out ( *p < 0.05 vs. control group, #p < 0.05 vs. E2 group ).

Article Snippet: The antibodies used during Western blots included rabbit anti-human ERα from Proteintech, rabbit anti-human ERβ (1:500) from Proteintech, mouse anti-human MMP-1(1:400) from Santa Cruz, mouse anti-human MMP-2(1:400) from Santa Cruz, rabbit anti-human MMP-9 (1:500) from Proteintech, rabbit anti-human p38MAPK(1:500)/p-p38MAPK(1:500) and rabbit anti-human tAkt (1:500)/pAkt(1:500) from Bioworld Technology.

Techniques: Western Blot, Expressing

Journal: Oncotarget

Article Title: Estrogen promotes tumor metastasis via estrogen receptor beta-mediated regulation of matrix-metalloproteinase-2 in non-small cell lung cancer

doi: 10.18632/oncotarget.16992

Figure Lengend Snippet: (A) After bilateral ovariectomy A549 cells (5 × 10 6 /100 μL) suspended in PBS were injected into 4-week-old female BALB/c nude mice via the tail vein, mice were randomly divided into five groups (N = 5/group): E2(0.09 mg/kg), PPT(1.05 mg/kg), DPN(1.89 mg/kg), E2+ Ful(1.46 mg/kg) and blank control. The lungs were removed after 4 weeks subcutaneously under drug treatment. Gross appearance of metastatic lung tumor nodes in different groups is indicated by bright yellow arrows. (B) Representative pathological image of metastatic A549 tumor at magnification 100ificatione pathological image of groups (N = 5/group): The number of metastatic nodules in the lungs (lung nodules number in every mouse of each group), metastatic index in different groups mentioned in ‘‘Materials and methods’’ section for the experimental. (C-E) The mean lung wet weight of each group, The number of metastatic nodules in the lungs (lung nodules number in every mouse of each group), metastatic index in different groups mentioned in ‘‘Materials and methods’’ section for the experimental. (F) Protein expression of ERβ, MMP-2, MMP-9, p-p38MAPK, p38MAPK, pAKT and tAKT in murine lung metastatic nodes was analyzed using Western blot, and (G) the detection of optical density. All data are expressed as the mean ± SD. Student's t-test was carried out ( *p < 0.05 vs. control group, #p < 0.05 vs. E2 group ).

Article Snippet: The antibodies used during Western blots included rabbit anti-human ERα from Proteintech, rabbit anti-human ERβ (1:500) from Proteintech, mouse anti-human MMP-1(1:400) from Santa Cruz, mouse anti-human MMP-2(1:400) from Santa Cruz, rabbit anti-human MMP-9 (1:500) from Proteintech, rabbit anti-human p38MAPK(1:500)/p-p38MAPK(1:500) and rabbit anti-human tAkt (1:500)/pAkt(1:500) from Bioworld Technology.

Techniques: Injection, Expressing, Western Blot

Journal: Poultry Science

Article Title: Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways

doi: 10.1016/j.psj.2024.103696

Figure Lengend Snippet: Information about primary antibodies.

Article Snippet: p-p38MAPK , 1:2000 , A1459 , ABclonal , China.

Techniques: